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Importantly, animals were singly housed to eliminate the exchange and cross-contamination of intestinal microbiomes, a factor that potentially biases studies with group-housed animals by minimizing microbiome variations for co-housed animals within a cohort

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Peptide degradation pathways Tirzepatide is a 39-amino-acid peptide with specific structural modifications that make it effective, including alpha-aminoisobutyric acid (Aib) substitutions at positions 2 and 13 (which protect against DPP-4 enzyme degradation in the body) and a C20 fatty diacid chain at position 20 (which enables albumin binding for a longer half-life)