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Determination of Nitric Oxide (NO) Production The nitrite (NO 2 ) was estimated colorimetrically at 12 and 24 h post CLP on the basis of reduction of nitrate to nitrite using Griess method ( Bacterial Load Determination For determination of colony-forming units (CFU) in blood and peritoneal fluid of mice ( n = 6 per/group), 10 L of samples were diluted with sterile Phosphate-Buffered Saline (PBS) 1:10, and then 10 L of each dilution was cultured in Mller Hinton Agar and incubated at 37 C for 24 h