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Oxidation of disulfide bonds of Pt GSTF8 Disulfide bonds between C13 of purified apo- Pt GSTF8 variants and GSH were formed by incubating 20 M Pt GSTF8 with 0 mM or 1 mM GSSG in the presence of 200 M GSH in 10 mM MOPSO, pH 6.45 for 2 h at 30 C, 800 rpm using a 3 mm shaking diameter
Coskun and Urva (2022, pharmacological description) describe the engineering: modified sequence derived from native GIP, Aib2 and Aib13 substitutions resisting DPP-4, and a C20 diacid fatty-acyl chain grafted to a lysine via a gamma-glutamate linker to extend half-life to approximately 6 days
No, they raise a hypothesis that there may be an association that justifies further research, but no cause-and-effect relationship can be determined