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The cell pellet was thawed, homogenized, and lysed in a buffer containing 20 mM HEPES pH 7.5, 50 mM NaCl, 2 mM MgCl 2 , and complete protease inhibitor cocktail tablet (Roche), and the membranes were collected by centrifugation at 40,000 g

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GI mucosal support: Because it is derived from gastric biology, it is frequently used in gut-lining support strategies

[61] found that peritubular myoid cells, Leydig cells, and Sertoli cells are the major storage sites of cytoplasmic ferritin in the testis and proposed the concept of compartmentalization of iron metabolism proteins to explain the maintenance of spermatogenesis (Fig