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[DOI] [PMC free article] [PubMed] [Google Scholar] 19.Lv X., Meng G., Li W., Fan D., Wang X., Espinoza-Pinochet C.A., Cespedes-Acua C.L

In addition, the absence or decrease in levels of cytosolic GSH resulted in PDI becoming more oxidized, limiting the ability of the enzyme to isomerize non-native disulphide bonds

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The mixture is then passed through a fresh glutathione resin column to remove the cleaved GST tag, allowing the purified target protein to be collected in the flow-through