After perfusion, the mouse brain was taken out carefully, and immersed in 4% paraformaldehyde solution overnight, then followed with 20% and 30% sucrose solution at 4 C for dehydration until isotonic
The injection guide walks through the mechanics for anyone who has never done a subcutaneous shot
Tert-Butyl hydroperoxide (TBHP), 27-Dichlorodihydrofluorescein diacetate (DCFDA), dimethyl sulfoxide (DMSO) for molecular biology, N-acetyl-L-cysteine (NAC), Pluronic F127, paraquat dichloride dihydrate, tannic acid, ethylenediaminetetraacetic acid (EDTA), ascorbic acid/sodium-L-ascorbate, sodium hydroxide (NaOH), hydrochloric acid (HCl), Mayers hematoxylin, and Eosin Y alcoholic solution were purchased from Sigma Aldrich (St
Candida Administration Worsens Uremia-Induced Gut Leakage in Bilateral Nephrectomy Mice, an Impact of Gut Fungi and Organismal Molecules in Uremia