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For Ube2d3-His 6 , the gene was cloned into pGEX-4T1 for expression as an N-terminal GST fusion with a TEV cleavage site
For the purpose of finding an effective and safe whiting substance which can be applied for preventing human hyperpigmentation, the natural product T1 is of great candidacy for evaluating its cell viability and inhibition of melanogenesis
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