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The mixture containing 250 L of 0.12 M sodium phosphate buffer, pH 8.0, 50 L of 0.5 M sodium sulfite (Sigma-Aldrich, Darmstadt, Germany), 50 L of 0.15 M D,L-dithiothreitol (DTT, Sigma-Aldrich, Darmstadt, Germany), 50 L of distilled water, 50 L of the cell homogenate, and 50 L of 0.1 M 3-mercaptopyruvate acid sodium salt (Sigma-Aldrich, Darmstadt, Germany), was incubated for 15 min at 37 C
Without controlled human studies, any dosing discussion is speculative and should be considered unsafe outside research settings
It uses a technique called liquid chromatography-high resolution mass spectrometry (LC-HRMS) to identify and measure the amount of ipamorelin in the blood sample
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