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step two, adding the solid phase resin, reacting for 24 hours at 80 in N-methylpyrrolidone, carrying out suction filtration, washing the solid phase resin with DCM, adding a DCM solvent of 25% TFA, and reacting for 2 hours at room temperature to obtain the 1- (2-hydroxyethyl) imidazole solid phase resin without tert-butyl protection or the 1- (3-hydroxypropyl) -1H-imidazole solid phase resin without tert-butyl protection

The ECA flippase WzxE flips the resulting ECA unit across the inner membrane prior to ECA maturation 57 (Fig

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