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As much of the PBS was removed as possible (the hemocyte pellet is not visible and is loosely attached on the Eppendorf tube) prior to storing the samples at -80 C

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Acknowledgements The authors would like to thank the management of the College of Pharmaceutical Sciences, Dayananda Sagar University, Harohalli for providing the scholarship to the research scholar for the Ph.D study

But even with these, the general consensus is to create the best environment for them to work, which means no alcohol immediately before or after