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DZN's inhibition of acetylcholinesterase (AChE) increases sympathetic nervous system activity and indirectly causes hepatic stress, and induction of oxidative stress (OS) leads to the generation of free radicals such as reactive oxygen species (ROS) contributes to damage cellular lipids, proteins, and DNA, leading to lipid peroxidation, nitric oxide reaction, and mitochondrial damage, which in turn affects the membrane permeability and allows the enzymes to leak into circulation

NAPQI) by cytochrome P450 enzymes (McGill and Jaeschke, 2013)