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There were also large increases in tin and bismuth (which are less toxic), some increases in mercury, thallium, antimony, and tungsten, and possible increases in uranium and (in later rounds) nickel

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364 The affected astrocytes demonstrate profound loss of function, in particular impaired K + buffering, 365,366 compromised H + transport, 367 and substantial decrease in glutamate uptake

Materials and methods Cell culture The K-562 and Lenti-X 293T cells were purchased from the American Type Culture Collection (CCL-243, ATCC) and Clontech (#632180), respectively