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Subsequently, At GSTF12 was shown to bind the anthocyanins cyanidin and cyanidin-3- O -glycoside, but was unable to conjugate them with GSH ( At GSTF12 is an anthocyanin transporter protein, or ligandin, able to transport these pigments for deposition in the vacuole, through its concerted action with TT12, a tonoplast-localized flavonoid/H+-antiporter ( At GSTF12 has orthologous functions to other GSTF genes involved in flavonoid accumulation namely, AN9 in petunia ( Fl3 in carnation ( VvGST4 in grapevine ( The observation that At GSTF12 has a non-enzymic ligand transport, or ligandin function has prompted us to look for binding associations for these proteins in plants, using ligand fishing to identify associations with natural products
Dual-luciferase reporter assay The 2000 bp promoter sequence of VEGFA and IL8 was searched on the UCSC website ( Based on the obtained promoter sequence, we predicted the possible signal transducer and activator of transcription 6 (STAT6) transcription factor-binding region of the VEGFA and IL8 promoter sequences
Activation of this receptor influences pathways associated with protein synthesis, cell survival, and structural adaptation
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