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Generally, protein purification entails five essential steps, i.e., extraction from biological matter, separation from lipids and nucleic acids (non-protein components), precipitation step, recovery of bulk protein from raw extract, utilization of hydrophobic or ion-exchange chromatography column for the separation of target protein from bulk protein, and more refined steps including affinity matrix to obtain highly refined target protein with high yields

Roan JN, Hsu CH, Fang SY, et al

The peptide triggers adaptations resembling exercise training responses even in the absence of physical activity, though most applications combine MOTS-c with exercise for synergistic effects

272 In addition, BCAT isoenzymes may serve as markers of tumor TME status