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Mice with no access to food during the activity phase (dark phase) of a 12-h light/12-h dark cycle had a significantly slower tumor progression and higher survival compared with mice that had no access to food during the inactivity phase (light phase), and mice that had access to food ad libitum 13

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For PDX tumors, frozen sections were established and stained with 10 M DCFH-DA and 20 M BODIPY 581/591 C11 for 30 min, and then washed twice by PBS