Furthermore, on encounter with a stress stimulus, a ROS-linked peculiar signal obstructs both cellular division and proliferation
After confirming that the concentration of NADPH in our lysates was within the linear detection range of the assay (Fig 3A), we treated LPS-stimulated (6 h) RAW264.7 Ms with 2-DG and diamide and quantified NADPH kinetics by obtaining lysates at 5-minute intervals for a total of 25 minutes (Fig 3B)
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This may be due to the presence of other particles formed during the treatment of liquids (e.g., hydrogen ions, peroxynitrites, superoxide, etc.) which, among other things, also lower the pH value (see Table 3)
