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Indeed, the transcription activity of the ARE motif was significantly induced by DMF treatment while ARE motif mutants showed reduced activities (Supplementary Fig

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Cells were collected by first quenching Trypsin 1:1 (v/v) with supplemented media and transferred to 15 ml Falcon tubes, followed by centrifugation (5 min, 2000 g , 20C)

Clinical Note: While glutathione supports many physiological processes, individual responses vary based on baseline health status, genetic factors affecting glutathione metabolism, environmental toxin exposure, and concurrent lifestyle factors