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NK Cell Cytotoxicity Impairment Natural Killer cells provide first-line immune surveillance without prior antigen sensitisation, making their function critical in immune defence against diabetes

Through systematic fractionation of human albumin, Pickart and colleagues isolated a small peptide responsible for the difference, eventually identifying it as a three-amino-acid sequence glycine, histidine, and lysine with an unusually strong affinity for binding copper(II) ions

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The supernatant was passed through a mouse anti-human AIM IgG monoclonal antibody (CL7)-immobilized HiTrap NHS-activated HP column and the protein was eluted with 0.1 M glycine-HCl (pH 2.5) and neutralized with 1 M Tris-HCl (pH 8.5)