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Potassium showed the widest gap: 95% of non-users fell short, compared to 71% of short-term users, meaning even the non-user group struggles here, but short-term GLP-1 users struggle more

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Thus, reads containing unique combinations of barcode, PCR tag, and IS were entered into the barcode/IS map for each Tn5 library, each representing a distinct DNA fragment (but also machine errors in sequencing, chimeric PCR amplicons, 3 truncations etc.)

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