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Sniffer-patch recordings For sniffer patch recordings, 13 M resistance fire-polished borosilicate glass electrodes with tips coated with refined beeswax were used and filled with internal pipette solution containing (in mM): CsCl (125), Tris-creatine-PO 4 (5.4), EGTA (10.0), HEPES (5.0), CaCl 2 (2.0), MgCl 2 (1.0), pH 7.4, 281 mOsm kg 1

The two coronavirus sites with exact P4-P1 matches to GCLC are those at either end of nsp5, i.e., M pro itself, which have the highest catalytic efficiency for cleavage of any of the viral M pro targets (100 and 41%)