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Samples containing identical amounts of protein were resolved using SDS-PAGE (Criterion, BioRad Laboratories), transferred to nitrocellulose, and blocked in Tris-buffered saline (TBS) containing 5% milk for 1 h at room temperature

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For kroniska tillstand eller allmant vavnadsunderaahll ar 6-veckorscykler med 2--4 veckors pauser vanliga