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Protein S -glutathionylation is recognized as an efficient regulator of redox signal transduction largely due to the high cellular concentrations of GSH and the reversibility of the reaction catalyzed by the glutaredoxin enzyme family [[27], [28], [29]]

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The novel use of the internal reference, LR3, spiked into the analytical samples allows for the entire workflow of the assay, sample preparation to LC-MS/MS detection, to be monitored and provides quality assurance of the assay performance

And yet, as noted in the opening paragraph of this perspective, despite all of this, APAP remains in the 20 th World Health Organization Model List of Essential Medicines.2 Conflicts of interest There are no conflicts to declare