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Waiting until significant loss has occurred reduces potential for recovery

This grain-free kibble is specially designed for older dogs and contains wild-caught, sustainable silver carp, which are high in protein and low in fat, which is especially important since senior sidekicks require more protein on a calorie basis than young adult dogs

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On day 6, cells were counted, and 100 l cell suspension (in DMEM media supplemented with only 10% FBS but no antibiotics) was added to the 96-well plates (containing siRNA and transfection mix) at the appropriate seeding density (12k for DMSO and 15k for etoposide-treated cells)