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Cell cycle arrest and chronic inflammation Senescent cells are characterized by irreversible cell cycle arrest, triggered by senescence-associated stressors, including DNA damage responses (DDRs) induced by oxidative stress, ROS, smoking, poor nutrition, oncogenes, gut microbiota, and mitochondrial dysfunction

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2.5 Liver tissue oxidant/antioxidant activity evaluation Following the protocol laid out earlier, the liver homogenate was produced (Ohkawa et al., 1979) and (Paglia and Valentine, 1967

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