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Then the unbound dye was removed (3000 rpm, 3 min, tabletop Elmi centrifuge), and the RBC precipitate was resuspended in 100 L of HEPES-buffer

Mechanistically, the principal ketone body 3-hydroxybutyrate (3HB) in a KD prevented the ICB-mediated upregulation of PD-L1 on myeloid cells while simultaneously promoting the expansion of CXCR3 + T cells

Other treatment options include an IV dose of acetyl-L-carnitine 5 mg/kg bolus loading dose followed by 0.025, 0.1, or 1 mg/kg constant infusion