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Given the complex interactions between the gut microbiome and systemic physiology, dysbiosis, characterized by altered microbial composition and reduced diversity, plays a significant role in the pathogenesis of HN [151]
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In HG-induced HK-2 and HFD/STZ-induced C57BL/6 mouse models, melatonin accelerates the movement of PINK1 and Parkin to mitochondria, activates mitophagy, reduces oxidative stress, and inhibits inflammation, thus exerting a protective effect in DKD [146]
Vectashield mounting medium (Vector Laboratories) was applied on the slide before the coverslip was placed and sealed with nail polish
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