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ME1 and ME3 require NADP + and ME2 utilizes either NAD + or NADP + for their catalytic activities, thus NADPH can be produced by ME both directly and indirectly through the activity of the NNT that catalyzes the transfer of hydride ions from NADH to NADP + and produces NADPH in mitochondria
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FIGURE 1 Rather than attempting confirmatory quantitative synthesis across all outcomes, we deliberately restricted quantitative analysis to a methodological demonstration
Step-by-Step Reconstitution Step 1: Clean Everything Protocols describe wiping the tops of both vials (CJC-1295 and BAC water) with alcohol swabs, then letting them air dry for 10 seconds
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