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Isolated CD4 + and CD8 + T cells were activated or not for up to 1, 3, or 6 days in a reverse time course with Dynabeads (1:1 cell-to-bead ratio, Gibco), magnetic beads similar in size to antigen-presenting cells and coupled with anti-CD3 and anti-CD28 antibodies, so that their total time in culture would be identical and to ensure that all cells would finish their indicated activation period the same day
A pesar de este incremento en el uso de esta herramienta, los resultados del tratamiento con bombas siguen siendo objeto de debate
For serious researchers, this makes it even more important to separate controlled data from uncontrolled reports
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