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Abbreviations BBD: Box-Behnken design BSA: Bovine serum albumin DTNB: (5,5-dithiobis-(2-nitrobenzoic acid) GPx: Glutathione peroxidase GSH: Reduced glutathione GSSG: Oxidized glutathione LOD: Limits of detection LOQ: Limits of quantitation RSM: Response surface modeling TBuOOH: Tert-Butyl hydroperoxide TNB: Nitro-2-thiobenzoate anion WSAVA: World Small Animal Veterinary Associations References Ademi M (2023) The role of ionized water and its impact on the activity of glutathione peroxidase on rats blood serum during hyperthermic stress

doi: 10.1038/nchembio.2238 52 YuanHLiXZhangXKangRTangD

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Cell death induction by curcumin Huh-7 cells treated with 25 M curcumin for 48 h and double-stained with YO-PRO-1 versus PI contained different populations, including viable cells (Y /PI ), apoptotic cells (Y + /PI or Y + /PI intermediate , i.e