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The B vitamins should not get near freezing temperatures

They may share the same active ingredient, but theyre prescribed as different brands for different usages

The samples were treated with tris (2-carboxyethyl) phosphine (10 L, 55 mM in 50 mM TEAB, 30 min, 60 C) followed by treatment with chloroacetamide (10 L, 120 mM in 50 mM TEAB, 30 min, 25 C in the dark)