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Polymerase chain reaction (PCR) for TNF-, IL-6, p16 and 18 s RNA (as an internal control) was also performed in duplicate using the following instruction and in a final volume of 20 L: (1) 10 L BIOFACT 2X Real-Time PCR Mix Master (for SYBR Green I
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Effect of n-3 and n-6 Fatty Acid Supplemented Diets on Semen Quality in Japanese Quail (Coturnix coturnix japonica)
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