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Based on the sequence requirements in penetratin for transduction, such as hydrophobicity, amphipathicity, basicity, and the chain length (Khafagy et al., 2010a), a modified peptide designated as shuffle (R,K fix) 2 (RWFKIQMQIRRWKNKK) that exhibits safe and enhanced insulin delivery was developed (Khafagy et al., 2010a)

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