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Int J Mol Sci 17(7):1006 Ko M-H, Yang M-L, Youn S-C, Lan C-T, Tseng T-J (2016) Intact subepidermal nerve fibers mediate mechanical hypersensitivity via the activation of protein kinase C gamma in spared nerve injury

2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

The semaglutide group lost an impressive average of 14.9% body weight over a 68 week period vs 2.4% in the placebo group