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Protein preparation and immunoblot analysis Whole rosettes from 4-week-old plants, which had been grown for 2 weeks under normal light followed by 2-week growth under high light, were collected and ground in liquid nitrogen, after which ~60 mg of the powder was homogenized with 500 L Extraction Buffer [150 mM Tris-HCl (pH 6.8), 7.5% -mercaptoethanol, 3% sodium dodecyl sulfate, and half a cOmplete mini protease inhibitor tablet (Roche)]

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The finding that GCLc- or GSS-deficient mice are non-viable in the embryonic period [35,36], while GCLm-deficient mice are viable and fertile with decreased GSH levels in the tissues compared to those of wild-type mice [37], suggest that GSH is essential for embryogenesis

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X., Ma, V., Mathey-Andrews, C

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