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It has been used extensively in animal studies, human cell models, and increasingly in off-label therapeutic settings, with minimal toxicity or organ stress reported

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Older people have a relative decrease in skeletal muscle mass and tend to have a smaller apparent volume of distribution of digoxin (which binds to muscle proteins)

For researchers in younger demographics, the same protocols apply, though the necessity of telomerase activation in individuals with adequate telomere length remains an open question