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Blood parameters evaluation All serum samples from Groups A and B were prepared and stored at 80C after centrifugation at 1500 g for 10 min and the levels of vitamin D3 and ferritin were measured using an automated biochemistry instrument (Cobas e411/Roche Company, Switzerland) according to the manufacturers instructions, whereas an automated hematology analyzer (Swelab alfa/Boule Medical AB, Sweden) was used to measure Hb levels

Membranes were exposed to image plates (BioMax, Kodak) for 5 min

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