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Diisopropylfluorophosphate labeled with 32 P (DF 32 P) reacts with esterases, especially cholinesterase, and becomes firmly bound to the cell ( 14 )

5,5,6,6-tetrachloro1,1,3,3tetraethylbenzimidazoylcarbocyanine iodide (JC-1) dye, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and human lactoferrin ELISA kits were obtained from Abcam (Cambridge, UK)

Stimulation of MRGPRX2 by various agonists results in mast cell stimulation and degranulation mediated by multiple intracellular pathways including Gi, Gq, PI3K/IP3 and increases of intracellular calcium (Wang et al, 2022)