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Diab Res Clin Pr 2019;157:107843

Setting Five diabetology units and one clinical research center in Italy

Core analytical requirements include: Primary structure confirmation using LC-MS/MS to verify intact mass, sequence integrity and detect modifications Impurity profiling (e.g., sequence variants, degradation products) using high-resolution techniques such as UHPLC-HRMS Aggregation and molecular weight analysis using orthogonal methods like SEC-MALS and sedimentation velocity analytical ultracentrifugation (SV-AUC)

indicating that NAc dopamine is necessary to elicit responses for rewards when the effort required is increased