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LC separation was via an XDB C 18 column (4.6150 mm) using a water/acetonitrile (both containing 0.1% (v/v) formic acid) gradient at a flow rate of 0.5 ml/min

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However, the most definitive sign of degradation is a loss of expected efficacy or inconsistent results in your research application.

These neuronal activation patterns are attenuated or absent in RAMP1/RAMP3 knockout animals, further implicating AMYR and AMYR as the functional receptors mediating these central effects