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Research findings point to the capacity of busulfan to induce cellular senescence in various cell types, including mesenchymal stem cells, fibroblasts, and the cell lines of human osteosarcoma cells U2OS and MG63 [65,66,67]

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2 2.8.3 Widely targeted metabolomics analysis Frozen tissue specimens were homogenized and extracted using ice-cold methanol:water (7:3, v/v) supplemented with isotope-labeled internal standards,followed by multi-platform LC-MS/MS analysis