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In brief, normalized counts were calculated by dividing raw read counts by sized factors and fitted to a negative binomial distribution, followed by the generalized linear model (GLM) likelihood ratio test (61)

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Heavy Metals, Method I 231 : NMT 10 ppm Iron 241 : NMT 10 ppm Residue on Ignition 281 : NMT 0.1% Organic Impurities Procedure Mobile phase: 6.8 g/L of potassium dihydrogen phosphate with 2.02 g/L of sodium 1-heptane sulfonate

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