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Modulating a complex system carries the possibility of unintended effects that require careful study

In this study, when the protein profile of the liver and kidney was investigated with SDS-PAGE, MTX did not change the liver protein profile, but it caused a decrease in some protein bands in the kidney tissue

That observation has implications for conversion accuracy: the precision difference between underdosing at 4.5mg versus hitting 5mg exactly may matter more at lower doses than the difference between 14mg and 15mg at higher doses