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The binding of HGF to c-met was detected with biotinylated anti-HGF polyclonal Ab (1:500 dilution in the 0.1% BSA-TTBS for 3 h at 25C), TACS streptavidin-HRP conjugate (1:1600 dilution for 20 min), and TMB substrate solution

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The lipoic acid groups on lipoylated proteins have relatively higher affinity for Cu + ( K d~10 17 M) 202