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4b), including peaks at 3424 cm 1 (overlapping of OH and NH stretches), 2925 cm 1 (asymmetric CH2 stretching vibration from the pyranose ring), 1648 cm 1 (C = O asymmetric stretching), 1549 cm 1 (NH2 groups), 1382 cm 1 (C-H bending), 1321 cm 1 (NH wag of primary and secondary amine), 1155 cm 1 (COC linkage), 1074 cm 1 (P = O stretching), 896 cm 1 (antisymmetric stretching vibration of COC bridges assigned to the glucopyranose ring in the CS matrix), and 529 cm 1 (OH out-of-plane bending vibration)
An aliquot of 50 l of the collected sample was subjected to cytokine measurement via Luminex magnetic beads according to the Mouse LX-MultiDTM-31 protocol (Bio-Rad, Hercules, CA, USA
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However, the peptide characteristics that enable absorption through the gastric mucosa, including its stability in acidic environments and resistance to enzymatic degradation, suggest it should maintain activity when absorbed through nasal mucosa as well
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