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A total of 5 l of Annexin V-FITC and 5 l of propidium iodide were added to each well, were mixed by pipetting, and incubated at room temperature in the dark for 15 min

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In contrast with the circumscribed triggers of GWS, ME/CFS is associated with a number of potential infectious triggers, while Epstein-Barr virus (EBV) has been hypothesized as a contributor to FM