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Then cDNA synthesis was performed using Revertaid cDNA synthesis kit with random hexamer primers (Thermo Scientific), 1ng was used for RT-qPCR analysis of MT-CYB , MT-CO2 and MT-RNR1

The study did not include a non-smoking control group, so results specifically compare E-cigarette users to conventional cigarette smokers, not to non-smokers

Villanti said tobacco companies have been working hard at the state and local levels to fight policies related to menthol as well as flavored tobacco products