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After one week of acclimatization, 36 male Wistar rats were divided into six groups (n=6) as follows: Group 1: Young male rats (aged 4 months, body weight = 115 10 g) Group 2: Young male control rats (aged 4 months, body weight = 115 10 g) Group 3: Middle-aged male rats (aged 12 months, body weight = 180 10 g) Group 4: Middle-aged male control rats (aged 12 months, body weight = 180 10 g) Group 5: Old male rats (aged 24 months, body weight = 220 10 g) Group 6: Old male control rats (aged 24 months, body weight = 220 10 g) Groups 1, 3, and 5 were exposed to passive cigarette smoke for 15 minutes once every day for 30 days and had free access to food and water

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