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The first people to feel smokes short-term effects are those in sensitive groups, considered vulnerable to smoke in lower concentrations, such as those over 65 or with health conditions like asthma and COPD, as well as children and outdoor workers

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The reverse transcription reaction was then carried out using High Capacity RNA to cDNA Kit (Invitrogen, USA) and followed by qPCR performed with specific TaqMan gene expression assays for FLG , LOR , IVL , KLK7 , TGM1 , and RPL13A genes (all ThermoFisher Scientific, USA) according to the manufacturers recommendations

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