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After this period, keratinocytes at passage 2 from healthy donors were seeded on the dermal equivalent at 1.2 10 6 cells/equivalent to form the epidermal layer and cultured for seven days in a combination of DMEM with Hams F12 in a proportion of 3:1 (DMEMH) supplemented with 5% Fetal Clone II serum (Hyclone, Scarborough, ON, Canada), 5 g/mL insulin (Sigma, St
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