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Conjugation of the reactive intermediates to water-soluble molecules is accomplished by Phase II conjugation enzymes, which consist of many enzyme superfamilies, including: Sulfotransferases (SULT) UDP-glucuronosyltransferases (UGT) Glutathione S-transferases (GST) N-acetyltransferases (NAT) 6 Conjugation reactions not only require the water-soluble fraction that will be attached to the toxinsuch as sulfate in the case of sulfation or glucuronic acid in the case of glucuronidationbut it also uses a large amount of energy in the form of adenosine triphosphate (ATP)

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